Dichloroanilines are actively used in the synthesis of drugs and pesticides; however, these compounds have been found to exhibit toxic activity. This study aimed to investigate the ability of the mycelium of Fomitopsis pinicola, Ganoderma tsugae, Pleurotus ostreatus, and Schizophyllum commune to biotransform two compounds of dichloroanilines under controlled conditions. The results indicate that the biodegradation rates of the studied compounds ranged from 83.95% to 99.85%. The highest percentage was recorded for G. tsugae 2566, whereas the lowest percentage was observed for both studied strains of S. commune. Five metabolites were identified during the biotransformation of 3,5-dichloroaniline: 3,5-dichloronitrobenzene, 3,5-dichloroacetanilide, 3,5-dichlorophenol, 2-amino-4,6-dichlorophenol, and 4-amino-2,6-dichlorophenol. Three of these metabolites were found for the first time after biotransformation of the studied compounds by fungal mycelium. This is the first report on 4-amino-3,5-dichlorophenol obtained as a result of biotransformation of 2,6-dichloroaniline by fungal mycelium.

Supplementary Material. Supplementary Materials (S1–S3) are available on this website: ukrbotj82-06-594-S1.pdf (78 KB), ukrbotj82-06-594-S2.pdf (84 KB), ukrbotj82-06-594-S3.pdf (269 KB)

Keywords: acetylation, aminodichlorophenol, biodegradation, chromatography, filamentous fungi, xenobiotic

Full text: PDF (Eng) 380K

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