The study results of the effects of Zinc, Manganese and Copper sulfates and citrates on cytokinins content in mycelial biomass of the valuable medicinal mushroom Trametes versicolor, strain 353 from the IBK Mushroom Culture Collection of the M.G. Kholodny Institute of Botany, in culture are presented. Cytokinins were measured using high performance liquid chromatography on an Agilent 1200 LC chromatograph with a G 1315 B diode matrix detector. The addition of salts of these metals was shown to accelerate the mycelium growth. The most effective was Copper citrate, its introduction to the nutrient medium caused the biomass growth increase by almost 80%. In general, citrates affected the growth of T. versicolor 353 more effectively than sulfates. Metal compounds stimulated formation of active forms of hormones (trans-zeatin and zeatin riboside) and reduced the content of inactive forms (cis-zeatin and zeatin-O-glucoside). The most significant effect on cytokinins metabolism was produced by Zinc salts, under its effect the levels of trans-zeatin and zeatin riboside increased eightfold. Sulfates of the metals influenced synthesis of zeatin riboside more effectively than citrates. All studied salts reduced the level of cis-zeatin at least twice. Minor quantities of zeatin-O-glucoside detected in the control were not discovered in experiments with metals. Although the effect of the studied microelements on acceleration of the growth of mycelial biomass was generally accompanied by an increase in the content of active forms of cytokinins, quantitative dependencies between these two indices were not recorded.

Keywords: Copper, cytokinins, Manganese, microelements, Trametes versicolor, Zinc

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