The dynamics of PIP-transcripts accumulation in S. latifolium leaves during twenty-four hours was demonstrated using RT-PCR method. Sium latifolium plants were sampled in nature in the Psyol River (aquatic plants) and on the riverside (terrestrial plants) near Velyka Bagachka settlement (Poltava Region, Ukraine). The highest level of PIP-expression observed during 6 am to 12 pm in both aerial-aquatic and terrestrial plants, and it gradually decreased to the end of the daylight hours. We assume that PIP-aquaporin gene expression increase in the beginning of daylight hours is the result of increased demand of plant for water since the transpiration rate increased at this time.

Keywords: aquaporins, gene expression, water deficit

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