Bactericidal activity of alcohol tinctures and water extracts of fruit bodies of mushroom species was investigated. It was established that bactericidal activity was species-specific and not dependent on trophic group, nutritional value, taxonomic position etc. The highest rates of growth inhibition of test cultures were demonstrated by extracts of Boletus regius, Cantharellus cibarius, Catathelasma imperiale, Laetiporus sulphureus, Mycena leptocephala, Polyporus umbellatus, Russula rosea and tinctures of M. leptocephala. Antibacterial properties of aqueous and alcoholic infusions of the same fungal species varied in their inhibitory effect; the water extracts were largely more efficient.

Keywords: macrofungi, mushroom therapy, aqueous extract, alcohol tincture, trophic group

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1. Alresly Z., Lindequist U., Lalk M., Porzel A., Arnold N., Wessjohann L.A. Bioactive triterpenes from the fungus Piptoporus betulinus. Rec. Nat. Prod., 2016, 10(1): 103–108.
2. Ayzenberg V.L., Borisenko A.V., Zakharchenko V.A., Kurchenko I.N., Kapichon A.P., Burban A.F., Konovalova V.V. In: Modern Mycology in Russia. Proceedings of the 2nd Congr. of the Russian mycologists, Moscow: Nats. akad. mykol., 2008, vol. 2, 321 pp.
3. Babitskaya V.G., Shcherba V.V., Puchkova T.A., Bisko N.A. In: Biologicheskie osobennosti lekarstvennykh makromitsetov v kulture: Sb. nauch. trudov. Ed. S.P. Wasser, Kiev: Altpress, 2012, vol. 2, pp. 76–344.
4. Bashirova R.M., Shpirnaya I.A., Galkin E.G., Ibragimov R.I., Tsvetkov V.O. Vestnik Bashkirskogo un-ta, 2014, 19(4): 1219–1222.
5. Bisko N.A., Lomberg M.L., Mikhailova O.B., Mitropolskaya N.Yu. In: Sovremennaya mykologiya v Rossii: materialy III Mezhdunar. Mikol. foruma, Moscow: Nats. Akad. Mikol., 2015, vol. 4, pp. 101–103.
6. Chen W., Zhao Z., Chen S.-F., Li Y-Q. Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro. Bioresour. Technol., 2008, 99: 3187–3194. https://doi.org/10.1016/j.biortech.2007.05.049 https://www.ncbi.nlm.nih.gov/pubmed/17624770
7. Chervona knyha Ukrainy. Roslynnyi svit (Red Data Book of Ukraine. Vegetable Kingdom). Ed. Ya.P. Didukh, Kyiv: Globalkonsaltyng, 2009, 912 pp.
8. Czygan F.C., Grunsfelder M. Carotenoids of the porter-lincoln-series in Anthurus archeri. Z. Naturforsch., 1975, 30: 297.
9. Diakov M.Yu., Kamzolkina O.V., Shtaer O.V., Bisko N.A., Poedinok N.L., Mikhailova O.B. Immunopatol., allergol., infektol., 2010, 1: 248.
10. Diakov M.Yu., Kamzolkina O.V., Shtaer O.V., Bisko N.A., Poedinok N.L., Mikhailova O.B. Mikol. i fitopatol., 2010, 44(3): 225–239.
11. Dugler B., Gonus A., Gucin F. Antimicrobial activity of the macrofungus Cantharellus cibarius. Pakistan J. Biol. Sci., 2014, 7(9): 1535–1539.
12. Dutta A.K., Chakraborty N., Pradhan P., Acharya K. Phallales of West Bengal, India. II. Phallaceae: Phallus and Mutinus. Researcher, 2012, 4(8): 21–25.
13. Dzyhun L.P., Kudrinetska A.B., Duhan O.M. Visnyk Natsionalnoho un-tu Lvivska politekhnika, 2011, 70: 156– 160.
14. Dzyhun L.P. Naukovi visti NTUU KPI, 2011, 3: 40–49.
15. Falandysz J., Kunito T., Kubota R. Selected elements in Brown Birch Scaber Stalk Leccinum scabrum. J. Environ. Sci. and Health., Pt A, 2007, 42: 2081–2088.
16. Garnweidner E. Mushrooms and Toadstools of Britain and Europe, London: Harper Collins Publ., 1994, 255 pp.
17. Giovannini I.S. Cultivated basidiomycetes as a source of new products: In vitro cultivation development, – selection of strains resistant to Trichoderma viride, – search for new active compounds, – factors influencing plasticity in Grifola frondosa: Thèse présentée ... Université de Neuchâtel, 2006, 210 pp.
18. Grytsay R.V., Varbanets L.D. Biotekhnolohiya, 2011, 4(1): 41–59.
19. Hall I.R., Stephenson S., Buchanan P., Wang Y., Cole A.L. Edible and Poisonous Mushrooms of the World, Portland: Timber Press, 2003, 371 pp.
20. Hawksworth D.L. Ainsworth & Bisby`s Dictionary of the Fungi, 8th ed. Oxon, Wallingford: CAB International, 1995, 616 pp.
21. Ivanova T.S., Bisko N.A., Barshtein V.Yu., Krupoderova T.A. Problemy kharchuvannya, 2010, 1–2: 42–47.
22. Johnson S.D., Jürgens A. Convergent evolution of carrion and faecal scent mimicry in fly-pollinated angiosperm flowers and a stinkhorn fungus. South Afr. J. Bot., 2010, 76(4): 796–807. https://doi.org/10.1016/j.sajb.2010.07.012
23. Kibby G. Atlas gribov: Opredelitel vidov, St. Petersburg: Amphora, 2009, 269 pp.
24. Kosani M., Rankovic B.M., Dasic M. Antioxidant and antimicrobial properties of mushrooms. Bulg. J. Agricult. Sci., 2013, 19(5): 1040–1046.
25. Kouassi K.A., Kouadio E.J., Hubert K.K., Edmond D.A., Patrice K.L. Phenolic compounds, organic acid and antioxidant activity of Lactarius subsericatus, Cantharellus platyphyllus and Amanita rubescens, three edible ectomycorrhizal mushrooms from center of Côte d'Ivoire Eurasian. J. Anal. Chem., 2016, 11(3): 127–139.
26. Kozarski M.S., Klaus A.S., Nikšić M.P., Griensven L.J., Vrvić M.M., Jakovljević D.M. Polysaccharides of higher fungi: biological role, structure and antioxidative activity. Hem. Ind., 2014, 68(3): 305–320. https://doi.org/10.2298/HEMIND121114056K
27. Kudryavets Ye., Lomberg M. In: Naukovi zdobutky molodi – vyrishennyu problem kharchuvannya lyudstva u XXI stolitti: prohrama i materialy 80 mizhnar. nauk. konf. molodykh uchenykh, aspirantiv i studentiv, Kyiv: NUKhT, 2014, part 1, pp. 620–622.
28. Kuka M., Cakste I., Galoburda R., Sabovics M. Chemical Composition of Latvian Wild Mushroom Cantharellus cibarius. In: Proceed. of the 9th Baltic Conf. on Food Science and Technology, FOODBALT 2014, Jelgata, Latvia, pp. 248–252.
29. Kuka M., Cakste I. Bioactive compounds in Latvian wild edible mushroom Boletus edulis. In: Proceed. of the 6th Baltic Conf. on Food Science and Technology, FOODBALT 2011, Jelgata, Latvia, pp. 116–120.
30. Leala A.R., Barrosa L., Barreiraa J., Martins A., Ferreiraa I. Antioxidant and bioactive compounds of two wild edible mushrooms from Northeast of Portugal: Boletus porosporus and Boletus regius. In: 1st Symp. on Medicinal Chemistry of University of Minho, Braga Campus de Gualtar, 2013, p. 5.
31. Marchenko M.M., Shmarakov I.A., Pasaylyuk M.V. Voprosy pitaniya, 2008, 77(6): 4–8.
32. Moliszewska E. Mushroom flavor. Folia Biologica et Oecologica, 2014, 10: 80–88. https://doi.org/10.2478/fobio-2014-0007
33. Pasaylyuk M.V. Biolohichno aktyvni rechovyny hrybiv. In: Litopys pryrody. Ed. Yu.P. Stefurak, Kosiv, 2015, vol. 12, pp. 200–212.
34. Patel S., Goyal A. Recent developments in mushrooms as anti-cancer therapeutics: a review. 3Biotech, 2012, 2: 1–15.
35. Poedinok N.L. Biotekhnologicheskie osnovy intensifykatsii kultivirovaniya sedobnykh i lekarstvennykh makromitsetov s pomoshchyu sveta nizkoy intensivnosti: Dr. Sci. Diss. Abstract, Kiev, 38 pp.
36. Petrychuk Yu.V., Pasaylyuk M.V., Sukhomlyn M.M. In: Roslynnyi svit u Chervoniy knyzi Ukrainy: vprovadzhennya Hlobalnoi stratehii zberezhennya roslyn: mat. III Mizhnar. nauk. konf., Lviv, 2014, pp. 231–234.
37. Sadovnikov N.V., Pridybaylo N.D., Vereshchak N.A., Zaslonov A.S. Obshchie i spetsialnye metody issledovaniya krovi ptits promyshlennykh krossov, Ekaterinburg; St. Petersburg: Uralskaya GSKhA, NPP AVIVAK, 2009, 85 pp.
38. Solomko E.F. Pishchevaya tsennost i lekarstvennye svoystva kultiviruemykh bazidialnykh makromitsetov. In: Biologicheskie osobennosti lekarstvennykh makromitsetov v culture: Sbornik nauch. trudov (Biological features of medicinal macromycetes in the culture: Digest of scientific works in two volumes). Ed. S.P. Wasser, Kiev: Alterpress, 2011, vol. 1, pp. 5–82.
39. Stijve T. Close encounters with Clathrus ruber, the latticed stinkhorn. Austral. Mycol. Newsletter, 1997, 16: 11–15.
40. Strugała P., Bieniek K., Sobczyk K., Rutkowska-Nowacka P., Grzywnowicz K. Can you become an allergy-man during a mushrooming? In: Youth and Progress of Biology: Abstracts of the VII Int. sci. conf. of students and postgraduate students, Lviv, 2011, p. 412.
41. Suprun S.M., Donchenko H.V., Parhomenko Yu.M., Kharkevich E.S., Kurchenko I.N., Aretinskaya T.B., Stepanenko S.P., Kravchenko O.A. Faktory eksperymentalnoi evolyutsii orhanizmiv, 2014, 15: 242–246.
42. Vahidi H., Kobarfard F., Namjoyan F. Effect of cultivation conditions on growth and antifungal activity of Mycena leptocephala. Afr. J. Biotech., 2004, 3(11): 606–609.
43. Wang D, Sun S.Q., Wu W.Z, Yang S.L, Tan J.M. Characterization of a water-soluble polysaccharide from Boletus edulis and its antitumor and immunomodulatory activities on renal cancer in mice. Carbohydrate Polymers, 2014, 105(25): 127–134. https://doi.org/10.1016/j.carbpol.2013.12.085 https://www.ncbi.nlm.nih.gov/pubmed/24708961
44. Wang J.C., Hu S.H., Su C.H., Lee T.M. Antitumor and immunogen handing activities of polysaccharide from culture broth of Hericium spp. Kaohsiung. J. Med. Sci., 2001, 17: 461. https://www.ncbi.nlm.nih.gov/pubmed/11842649
45. Wasser S.P. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides.Appl. Microbiol. Biotechnol., 2002, 60: 258–274. https://doi.org/10.1007/s00253-002-1076-7 https://www.ncbi.nlm.nih.gov/pubmed/12436306
46. Woodward A., Kell D. On the relationship between the nonlinear dielectric properties and respiratory activity of the obligately aerobic bacterium Micrococcus luteus. Bioelectrochem. and Bioenerg., 1991, 26: 423–439. https://doi.org/10.1016/0302-4598(91)85005-M
47. Yang S.P., Xu J., Yue J.M. Sterols from the fungus Catathelasma imperiale. Chinese J. Chem., 2003, 21(10): 1390–1394. https://doi.org/10.1002/cjoc.20030211031
48. Yansen P. Vse o gribakh, SZKEO: Kristall, 2004, 161 pp.
49. Zavodovskiy P.G. Afilloforoidnye griby Vodlozerya, Petrozavodsk: Petrozavod. gos. un-t, 2011, 68 pp.
50. Zerova M.Ya., Radzievskyi G.G., Shevchenko S.V. Vyznachnyk hrybiv Ukrainy. Bazydiomitsety. Knyha 1. Ekzobazydialni, Afiloforalni, Kantarelalni (Handbook of the Fungi of Ukraine. Basidiomycetes. Book 1. Exobasidiales, Aphyllophorales, Cantharellales), Kyiv: Naukova Dumka, 1972, vol. 5, 240 pp.
51. Zerova M.Ya., Sosin P.Ye., Rozhenko G.L. Vyznachnyk hrybiv Ukrainy. Bazydiomitsety. Knyha 2. Boletalni, strobilomitsetalni, trikholomatalni. entolomatalni, rusulalni, agarikalni, gasteromitsety (Handbook of the Fungi of Ukraine. Basidiomycetes. Book 2. Boletales, Strobilomycetales, Tricholomatales, Entolomatales, Russulales, Agaricales, Gasteromycetes), Kyiv: Naukova Dumka, 1979, 565 pp.
52. Zhang G., Zeng X., Li C., Li J., Huang Y., Han L., Wei J.A., Huang H. Inhibition of urinary bladder carcinogenesis by aqueous extract of sclerotia of Polyporus umbellatus fries and polyporus polysaccharide. Amer. J. Chin. Med., 2011, 39(1): 135–144. https://doi.org/10.1142/S0192415X11008701 https://www.ncbi.nlm.nih.gov/pubmed/21213404
53. Zjawiony J.K. Biologically active compounds from Aphyllophorales (Polypore) fungi. J. Nat. Prod., 2004, 67: 300–310. https://doi.org/10.1021/np030372w https://www.ncbi.nlm.nih.gov/pubmed/14987072