Ukr. Bot. J. 2015, 72(2): 180–185 https://doi.org/10.15407/ukrbotj72.02.180Plant Physiology, Biochemistry, Cell and Molecular Biology
In vitro Arabidopsis thaliana root anatomy and ultrastructure under clinorotation
Bulavin I.V.- M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv
- 2, Tereshchenkivska Str., Kyiv, 01001, Ukraine
Abstract
Anatomical and ultrastructural patterns of roots formed in vitro on leaf explants in control and under clinorotation are described. It was shown that there is no significant differences in length of root growth zones, their cell number and size. Graviperceptive cells in a root cap are differentiated under clinorotation, likewise in the control; however, they do not function in these conditions. A mitochondrion size decreases in meristem cells under clinorotation that is an evidence of these organelle sensitivity to altered gravity conditions. We proposed the model of rhizogenesis in vitro for performing the space experiments to study the importance of a gravity scalar value to start the program of graviperceving cell differentiation.
Keywords: clinorotation, in vitro roots, anatomy, ultrastructure
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