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Ukr. Bot. J. 2019, 76(6): 486–492
https://doi.org/10.15407/ukrbotj76.06.486
Fungi and Fungi-like Organisms

Micromorphological features of species of Ganoderma (Ganodermataceae) in pure culture

Boromenskyi D.O., Bisko N.A.
Abstract

Mycelial microstructures of taxa of the genus Ganoderma from the IBK Culture Collection of Mushrooms at the M.G. Kholodny Institute of Botany NASU were investigated by light microscopy and scanning electron microscopy. In total, seven strains of the following five species were studied: G. carnosum, G. resinaceum, G. sinense, G. tsugae, and G. oregonense. Mycelium of all these species consists of thin-walled septate hyphae of different size. Characteristic microstructures for vegetative mycelia were observed: clamp connections, swollen hyphae, mycelial pellicle, mycelial cords, staghorn (coralloid) hyphae, and chlamydospores. For G. carnosum the following mycelial microstructures were found: single clamp connections with or without anastomoses, double clamp connections with anastomoses and rarely swollen hyphae; for G. resinaceum and G. sinense – single clamp connections, large number of staghorn hyphae, sometimes with perforations; in addition, for G. resinaceum ellipsoid chlamydospores with smooth surface were observed. Mycelium of G. tsugae was found to have two kinds of clamp connections, single clamps with or without anastomoses while in mycelium of G. oregonense single clamp connections and occasionally swollen hyphae were seen. Double clamp connections and those with anastomoses were noticed for the first time in vegetative mycelium of G. carnosum, as well as staghorn hyphae – in the mycelia of G. sinense and G. carnosum.

Keywords: Ganoderma, mycelium, microstructures, macromycetes, scanning electron microscopy, pure culture

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References
  1. Adaskaveg J.E., Gilbertson R.L. 1986. Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex. Mycologia, 78(5): 694–705.
  2. Adaskaveg J.E., Gilbertson R.L. 1989. Cultural studies of four North American species in the Ganoderma lucidum complex with comparisons to G. lucidum and G. tsugae. Mycological Research, 92(2): 182–191.
  3. Balaes T., Tanase C. 2012. Description of in vitro cultures for some spontaneous lignicolous basidiomycetes species. Biologie vegetata, 58(2): 19–29.
  4. Belova N.V. 2016. Advances in Biology & Earth Sciences, 1(1): 111–120.
  5. Bisko N.A., Babitskaya V.G., Buchalo A.S., Krupodorova T.A., Lomberg M.L., Mychaylova O.B., Puchkova T.A., Solomko E.F., Shcherba V.V. 2011. Byolohycheskye svoystva lekarstvennykh makromytsetov v culture Ed. S.P. Wasser. Kiev: Alterpress, vol. 2, 459 pp.
  6. Bisko N.A., Lomberg M.L., Mytropolska N.Yu., Mychaylova O.B. 2016. The IBK Mushroom Culture Collection. Kyiv: Alterpress, 120 pp.
  7. Boh B., Berovic M., Zhang J., Zhi-Bin L. 2007. Ganoderma lucidum and its pharmaceutically active compounds. Biotechnology Annual Review, 13: 265–301. https://doi.org/10.1016/s1387-2656(07)13010-6
  8. Buchalo A.S., Didukh M.Ya. 2005. Micromorphological characteristics of culinary-medicinal mushroom and fungi cultures. International Journal on Medicinal Mushrooms, 7(1): 249–261.
  9. Buchalo A.S., Mykchaylova O.B., Lomberg M.L., Wasser S.P. 2009. Microstructures of vegetative mycelia of macromycetes in pure cultures. Kyiv: Alterpress, 214 pp.
  10. Buchalo A.S., Babitskaya V.G., Bisko N.A., Wasser S.P., Dudka I.A. Mitopolskaya N.Yu., Mykchaylova O.B., Negreyko A.M., Poyedinok N.L., Solomko E.F. 2011. Byologicheskie svoystva lekarstvennykh makromitsetov v culture. Ed. S.P. Wasser. Kiev: Alterpress, vol. 1, 212 pp.
  11. Douanla-Meli C., Langer E. 2009. Ganoderma carocalcareus sp. nov., with crumbly-friable context parasite to saprobe on Anthocleista nobilis and its phylogenetic relationship in G. resinaceum group. Mycological Progress, 8(2): 145–155. https://doi.org/10.1007/s11557-009-0586-4
  12. Jargalmaa S., Eimes J.A., Park M.S., Park J.Y., Oh S.-Y., Lim Y. W. 2017. Taxonomic evaluation of selected Ganoderma species and database sequence validation. PeerJ, 5: 1–16. https://doi.org/10.7717/peerj.3596
  13. Li-Ying L., Hui C., Chao L., Hong-Qing W., Jie K., Yan L., Ruo-Yun C. 2014. Triterpenoids of Ganoderma theaecolum and their hepatoprotective activities. Fitoterapia, 98: 254–259. https://doi.org/10.1016/j.fitote.2014.08.004
  14. Richter C., Wittstein K., Kirk P.M., Stadler M. 2014. An assessment of the taxonomy and chemotaxonomy of Ganoderma. Fungal Diversity, 71(1): 1–15. https://doi.org/10.1007/s13225-014-0313-6
  15. Tchoumi J.M.T., Coetzee M.P.A., Rajchenberg M., Roux J. 2019. Taxonomy and species diversity of Ganoderma species in the Garden Route National Park of South Africa inferred from morphology and multilocus phylogenies. Mycologia, 18(1): 730–747. https://doi.org/10.1080/00275514.2019.1635387
  16. Tsivileva O., Nguyen T., Vu L., Yurasov N., Chernyshova M., Petrov A., Galushka V., Markin A., Koftin O. 2016. Vietnamese Ganoderma: growth, peculiarities, and low-molecular composition compared to European and Siberian strains. Turkish Journal of Botany, 40(3): 269–286. https://doi.org/10.3906/bot-1410-15
  17. Quattelbaum D., Carner G.-R. 1980. A technique for preparing Beaveria spp. for scanning electron microscopy. Canadian Journal of Botany, 58: 1700–1703. https://doi.org/10.1139/b80-198
  18. Stalpers J.A. 1978. Identification of wood-inhabiting Aphyllophorales in pure culture. Studies in Mycology, 16(1): 1–248.
  19. Wasser S.P. 2010. Medicinal mushrooms Science: History, Current status, Future Trends and Unsolved Problems, International Journal on Medicinal Mushrooms, 12(1): 1–16.
  20. Wasser S.P. 2014. Medicinal Mushroom Science: Current Perspectives, Advances, Evidences, and Challenges. Biomedical Journal, 37(6): 345–35. https://doi.org/10.4103/2319-4170.138318
  21. Zmitrovich I.V. 2012. In: Mitselialnyi obraz zhyzni i ekologo-troficheskye gruppy gribov: Materialy VI Vserossyiskoy mikologicheskoy shkoly-konferentsyi s mezhdunarodnym uchastiem. Moscow, pp. 1–34.