Mycelial microstructures of taxa of the genus Ganoderma from the IBK Culture Collection of Mushrooms at the M.G. Kholodny Institute of Botany NASU were investigated by light microscopy and scanning electron microscopy. In total, seven strains of the following five species were studied: G. carnosum, G. resinaceum, G. sinense, G. tsugae, and G. oregonense. Mycelium of all these species consists of thin-walled septate hyphae of different size. Characteristic microstructures for vegetative mycelia were observed: clamp connections, swollen hyphae, mycelial pellicle, mycelial cords, staghorn (coralloid) hyphae, and chlamydospores. For G. carnosum the following mycelial microstructures were found: single clamp connections with or without anastomoses, double clamp connections with anastomoses and rarely swollen hyphae; for G. resinaceum and G. sinense – single clamp connections, large number of staghorn hyphae, sometimes with perforations; in addition, for G. resinaceum ellipsoid chlamydospores with smooth surface were observed. Mycelium of G. tsugae was found to have two kinds of clamp connections, single clamps with or without anastomoses while in mycelium of G. oregonense single clamp connections and occasionally swollen hyphae were seen. Double clamp connections and those with anastomoses were noticed for the first time in vegetative mycelium of G. carnosum, as well as staghorn hyphae – in the mycelia of G. sinense and G. carnosum.
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