The article provides results of our revision of three herbarium specimens of the genus Tulostoma collected in Ukraine and deposited in the Mycological Herbarium of V.N. Karazin Kharkiv National University, CWU (Myc). Previously, they have been identified as Tulostoma kotlabae, T. niveum, and T. subsquamosum and reported as newly recorded species in Ukraine. For the examined specimens, we analyzed the nucleotide sequences of the ITS region of ribosomal DNA. For one specimen, the large subunit (LSU) of rDNA was also obtained. Based on the nucleotide sequencing results, one specimen (GB00121) was re-identified as Tulostoma melanocyclum and the other two specimens (GB00125 and GB00129) – as T. simulans. The latter species is reported in Ukraine for the first time. Given that genus Tulostoma has been subject to a number of recent taxonomic changes, it would be reasonable to make a revision of other herbarium specimens from Ukraine using molecular methods.

Keywords: Crimea, DNA barcoding, Donetsk Region, floristic records, gasteroid fungi, Luhansk Region, Ukraine, Ukrainian Steppe Nature Reserve

Full text: PDF (Ukr) 664K

1. Abarenkov K., Tedersoo L., Nilsson R.H., Vellak K., Saar I., Veldre V., Parmasto E., Prous M., Aan A., Ots M., Kurina O., Ostonen I., Jõgeva J., Halapuu S., Põldmaa K., Toots M., Truu J., Larsson K.H., Kõljalg U. 2010. PlutoF – a web based workbench for ecological and taxonomic research, with an online implementation for fungal ITS sequences. Evolutionary Bioinformatics, 6: 189–196. https://doi.org/10.4137%2FEBO.S6271
2. Akata İ., Sahin E., Altuntaş D., Kabaktepe Ş. 2021. Two new additions to Turkish Tulostoma. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(5): 1104–1110. https://doi.org/10.18016/ksutarimdoga.vi.839465
3. Bobyak H. 1907. Zbirnyk matematychno-pyrodopysno-likarskoyi sektsii Naukovoho tovarystva imeni Shevchenka, 11: 1–40.
4. Czerniaiev B.M. 1845. Nouveaux cryptogames de l'Ukraine et quelques mots sur la flore de ce pays. Bulletin de la Société Impériale des Naturalistes de Moscou, 18(3): 132–157. https://biostor.org/reference/237667 and https://www.biodiversitylibrary.org/part/246972
5. Dörfelt H. 2005. Die Gasteromyceten der Mongolei und ihre Bedeutung für die Systematik, Chorologie und Ökologie. Erforschung biologischer Ressourcen der Mongolei 9: 465–474.
6. Esqueda M., Moreno G., Pérez-Silva E., Sánchez A., Altés A. 2004. The genus Tulostoma in Sonora, Mexico. Mycotaxon, 90(2): 409–422.
7. Forin N., Vizzini A., Nigris S., Ercole E., Voyron S., Girlanda M., Baldan B. 2020. Illuminating type collections of nectriaceous fungi in Saccardo's fungarium. Persoonia, 45: 221–249. https://doi.org/10.3767/persoonia.2020.45.09
8. Fungi of Ukraine. 1999–onward. Available at: www.cybertruffle.org.uk/ukrafung/ukr (Accessed 2 February 2022).
9. GBIF. 2022a. Tulostoma melanocyclum Bres. GBIF Occurrence Download. Available at: https://doi.org/10.15468/dl.ugy38a (Accessed 14 January 2022).
10. GBIF. 2022b. Tulostoma simulans Lloyd. GBIF Occurrence Download. Available at: https://doi.org/10.15468/dl.euvjmb (Accessed 14 January 2022).
11. Geesteranus M.R.A. 1971. De Nederlandse Tulostoma-soorten. Gorteria Dutch Botanical Archives, 5(7): 89–93.
12. Jeppson M., Altés A., Moreno G., Nilsson R.H., Loarce Y., de Bustos A., Larsson E. 2017. Unexpected high species diversity among European stalked puffballs – a contribution to the phylogeny and taxonomy of the genus Tulostoma (Agaricales). MycoKeys, 21: 33–88. https://doi.org/10.3897/mycokeys.21.12176
13. Kholfy S., Nmichi A., Ouabbou A., Ajana M., Belahbib N., Ouazzani T.A., Benkirane R., Douira A. 2017. Study of two fungal species of Tulostoma genus encountered for the first time in Morocco: Tulostoma melanocyclum Bres. and Tulostoma kotlabae Pouzar. International Journal of Environment, Agriculture and Biotechnology, 2(1): 235–39. https://doi.org/10.22161/ijeab/2.1.30
14. Kotlaba F., Zehnálek P. 2018. Sixteen gasteromycetes collected in Cuba 50 years ago. Czech Mycology, 70(2): 185–209. https://doi.org/10.33585/CMY.70206
15. Léveillé J.H. 1842. Observation médicales et énumération des plantes recueillies en Tauride. In: Voyage dans la Russie méridionale et la Crimée: par la Hongrie, la Valachie et la Moldavie, vol. 2. Paris: Ernest Bourdin et Co., pp. 33–242.
16. Linnaeus C. 1753. Species Plantarum, vol. 2. Holmiae: Imprensis Laurentii Salvii, pp. 560–1200.
17. Łuszczyński J. 2000. The first record of Tulostoma melanocyclum in Poland. Acta Mycologica, 35(1): 37–40. https://doi.org/10.5586/am.2000.004
18. Maslov I.I., Sarkina I.S., Belych T.V., Sadogurskyi S.E. 1998. Annotated catalogue of algae and fungi of the reserve "Mys Martyan". Yalta, 31 pp.
19. Moreno G. 2001. Notes on the genus Tulostoma in H. Kreisel's herbarium. Cryptogamie Mycologie, 22(1): 57–66. https://doi.org/10.1016/S0181-1584(01)01056-9
20. Pärtel K., Suija A., Yatsiuk I. 2021. The mycological collection of G.C. Girgensohn from the middle of the 19th century. Folia Cryptogamica Estonica, 58: 109–119. https://doi.org/10.12697/fce.2021.58.14
21. Persoon C.H. 1794. Neuer Versuch einer systematischen Einteilung der Schwämme. Neues Magazin für die Botanik, 1: 63–137.
22. Persoon C.H. 1801. Synopsis methodica fungorum, vol. 1 [Pars prima]. Göttingen: H. Dieterich, xxx + 240 pp. https://doi.org/10.5962/bhl.title.166151
23. Rusevska K., Calonge F. D., Karadelev M., Martín P. M. 2019. Fungal DNA barcode (ITS nrDNA) reveals more diversity than expected in Tulostoma from Macedonia. Turkish Journal of Botany, 43: 102–115. https://doi.org/10.3906/bot-1804-38
24. Smith J.H., Suz L.M., Ainsworth A.M. 2016. Red List of Fungi for Great Britain: Bankeraceae, Cantharellaceae, Geastraceae, Hericiaceae and selected genera of Agaricaceae (Battarrea, Bovista, Lycoperdon & Tulostoma) and Fomitopsidaceae (Piptoporus). Kew: Royal Botanic Gardens, 90 pp.
25. Sudhir K., Stecher G., Li M., Knyaz C., Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547–1549. https://doi.org/10.1093/molbev/msy096
26. Syvokon O.V. 2011. Gasteromycetes of the Left-Bank Ukraine. Cand. Sci. Diss. Abstract. Kyiv: M.G. Kholodny Institute of Botany, NAS of Ukraine, 24 pp.
27. Tomaszewska A., Łuszczyński J., Łuszczyńska B., Jaworska J. 2011. Taxonomic notes on the Polish Tulostoma species. Acta Mycologica, 46(2): 179–186. https://doi.org/10.5586/am.2011.011
28. Tournefort J.P. 1700. Institutiones rei herbariae. T. I. Parisiis [Paris]: E Typographia Regia, 698 pp. https://doi. org/10.5962/bhl.title.713
29. Tranzschel W.G. 1905. Contributiones ad floram mycologicam Rossiæ. Enumeratio fungorum in Tauria lectorum. Travaux du Musée Botanique de l'Académie Impériale des Sciences de St.-Pétersbourg, 2: 31–47.
30. Vilgalys R., Hester M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology, 172(8): 4238–4246. https://doi.org/10.1128/ jb.172.8.4238-4246.1990
31. White T.J., Bruns T., Lee S., Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, 18(1): 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
32. Wright J.E. 1987. The genus Tulostoma (Gasteromycetes) – A world monograph. In: Bibliotheca Mycologica, Bd. 113. Berlin, Stuttgart: J. Cramer, 338 pp.
33. Zerova M.Ya., Sosin P.E., Rozhenko G.L. 1979. Handbook of Fungi of Ukraine, vol. 5, book 2. Basidiomycetes: Boletales, Strobilomycetales, Tricholomatales, Entolomatales, Russulales, Agaricales, Gasteromycetes. Kyiv: Naukova Dumka, 556 pp.