Influence of liquid static and submerged cultivation conditions on production of biomass and endopolysaccharides in the mycelia of ten strains of seven species of the genus Ganoderma from the Mushroom Culture Collection (IBK) was investigated. The selected strains were cultivated in a liquid glucose-peptone-yeast medium for 14 days. For biomass production, submerged cultivation was more advantageous, as compared to liquid static cultivation, for all studied strains except G. oregonense 2560. Effect of the submerged cultivation method on the content of polysaccharides in the mycelium was demonstrated only for four investigated strains of Ganoderma (G. tsugae 2566, 2024 and G. resinaceum 2503, 2477). The strain G. oregonense 2560 contained the highest percentage of endopolysaccharides in the mycelium (8.2%) obtained using the method of liquid static cultivation. The highest mycelium biomass was produced in liquid static culture by the strains G. resinaceum 2477, 2503 (9.4 g/l), G. oregonense 2560 (9.3 g/l), and G. applanatum 1899 (9.0 g/l). The highest biomass value (20.7 g/l) and the highest yield of endopolysaccharides (1.58 g/l) were obtained in mycelium of G. tsugae 2024 in submerged culture. For G. tsugae and G. resinaceum, a strain specificity in endopolysaccharides content under submerged and static liquid cultivation was established. Data on the production of biomass and endopolysaccharides by the studied strains of G. carnosum and G. oregonense were obtained for the first time.

Keywords: Ganoderma oregonense, Ganoderma carnosum, Ganoderma sinense, liquid static cultivation, submerged cultivation

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