ISSN 2415-8860 (online), ISSN 0372-4123 (print)
logoUkrainian Botanical Journal
  • 2 of 9
Up
Ukr. Bot. J. 2019, 76(1): 9–23
https://doi.org/10.15407/ukrbotj76.01.009
Plant Taxonomy, Geography and Floristics

Pollen morphology of species of Dipsacus (Dipsacaceae) in the flora of Ukraine: significance for taxonomy and spore-pollen analysis

Tsymbalyuk Z.M., Bezusko L.G., Mosyakin S.L., Nitsenko L.M.
Abstract

Pollen morphology of six species of the genus Dipsacus (Dipsacaceae) in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains of these species are 3-porate, spheroidal, ellipsoidal or oblate-spheroidal; largesized. Their outline in equatorial view is circular or oval, in polar view circular-triangular. Pores are distinct, lolongate, circular and elliptical, with an annulus and operculum. Sculpture exine is spinate-spinulate. Additional characters of taxa of Dipsacus diagnostic at the species level for the purposes of taxonomy are the dimensions of pollen and pores, shape of pores, width of the annulus, structure of the operculum, structure of the exine, and the size and location of spines. The size of pollen and pores, shape of pores, and the width of the annulus are promising for the pollen identification in the spore-pollen analysis. The exine sculpture can be used for identification of pollen at the generic level. Palynomorphological data are partly consistent with the traditional system of genus. The significance of morphological features of pollen grains of representatives of the genus Dipsacus is more obvious at the species level. The available paleofloristic data on the participation of pollen grains of Dipsacaceae in the composition of subfossil spore-pollen spectra and palynofloras of the deposits of the Upper Pleistocene – Holocene of Ukraine are generalized. The high degree of fluctuation in participation of pollen of representatives of Dipsacus in paleofloristic material has been revealed. The generalized data allowed to substantiate the possibility and significance of the use of the presented palynomorphological data for taxa of Dipsacus of the flora of Ukraine in the practice of palynological studies of the Pleistocene and Holocene deposits.

Keywords: diagnostic characters, Dipsacaceae, Dipsacus, fossil palynoflora, Holocene, morphology, paleofloristics, pollen, taxonomy, Ukraine, Upper Pleistocene

Full text: PDF (Ukr) 12M

References
  1. Angiosperm Phylogeny Group III (APG III). 2009. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Botanical Journal of the Linnean Society, 161: 105–121. https://doi.org/10.1111/j.1095-8339.2009.00996.x
  2. Angiosperm Phylogeny Group IV (APG IV). 2016. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG IV. Botanical Journal of the Linnean Society, 181: 1–20. https://doi.org/10.1111/boj.12385
  3. Arap R.Ya. 1972. Ukrainian Botanical Journal, 29(4): 506–513.
  4. Arap R.Ya. 1974. Ukrainian Botanical Journal, 31(1): 493–498.
  5. Arap R.Ya. 1976. Palinologicheskie issledovaniya poverkhnostnykh sloev pochvy Ukrainskogo Polesya. In: Palinologicheskie issledovaniya osadochnykh otlozheniy Ukrainy I smezhnykh territoriy. Kiev: Naukova Dumka, pp. 11–16.
  6. Arap R.Ya. 1984. Ukrainian Botanical Journal, 41(1): 73–77.
  7. Artyushenko A.T. 1970. Rastitelnost Lesostepi i Stepi Ukrainy v chetvertichnom periode (po dannym sporovo-pyltsevogo analiza). Kiev: Naukova Dumka, 176 pp.
  8. Artyushenko A.T., Arap R.Ya., Bezusko L.G. 1982. Istoriya rastitelnosti zapadnykh oblastey Ukrainy v chetvertichnom periode. Kiev: Naukova Dumka, 136 pp.
  9. Artyushenko A.T., Pashkevich G.A., Parishkura S.I., Kareva E.V. 1973. Paleobotanicheskaya kharakteristika opornykh razrezov chetvertichnykh (antropogenovykh) otlozheniy sredney i yuzhnoy chasti Ukrainy. Kiev: Naukova Dumka, 96 pp.
  10. Backlund A., Donoghue M.J. 1996. Morphology and phylogeny of the order Dipsacales. In: Phylogeny of the Dipsacales, part 4. Ed. A. Backlund. Uppsala (Sweden): Uppsala Univ., pp. 1–55.
  11. Bell C.D., Donoghue M.J. 2005. Dating the diversification of Dipsacales: comparing models, genes, and evolutionary implications. American Journal of Botany, 92: 284–314. https://doi.org/10.3732/ajb.92.2.284 https://www.ncbi.nlm.nih.gov/pubmed/21652406
  12. Bell C.D., Edwards E.J., Kim S.-T., Donoghue M.J. 2001. Dipsacales phylogeny based on chloroplast DNA sequences. Harvard Papers in Botany, 6(2): 481–499.
  13. Bezusko L.G., Tsymbalyuk Z.M. 2011. Palynotheka of the M.H. Kholodny Institute of Botany, National Academy of Sciences of Ukraine. In: Herbaria of Ukraine. Index Herbariorum Ucrainicum. Ed. N.M. Shiyan. Kyiv: Alterpress, pp. 138–141.
  14. Bezusko L.H. 2014. Ukrainian Botanical Journal, 71(6): 694–701. https://doi.org/10.15407/ukrbotj71.06.708
  15. Bezusko L.H., Mosyakin S.L., Bezusko A.H. 2011. Zakonomirnosti ta tendentsii rozvytku roslynnoho pokryvu Ukrainy u piznomu pleystotseni ta holotseni. Kyiv: Alterpress, 448 pp.
  16. Bobrov E.G. 1957. Dipsacaceae. In: Flora SSSR, vol. 24. Eds B.K. Shishkin, E.G. Bobrov. Moscow; Leningrad: Izd-vo AN SSSR, pp. 10–91.
  17. Bolikhovskaya N.S. 1981. Rastitelnost i klimat Srednego Pridnestrovya v pozdnem pleystotsene. Rezultaty palinologicheskogo analiza otlozheniy Kishlyanskogo Yara. In: Ketrosy. Musterskaya stoyanka na Srednem Dnestre. Moscow: Nauka, pp. 103–124.
  18. Bolikhovskaya N.S. 1995. Evolyutsiya lessovo-pochvennoy formatsii Severnoy Evrazii. Moscow: Izd-vo Mosk. unta, 270 pp.
  19. Bremer K., Backlund A., Sennblad B., Swenson U., Andreasen K., Hjertson M., Lundberg J., Backlund M., Bremer B. 2001. A phylogenetic analysis of 100+ genera and 50+ families of euasterids based on morphological and molecular data with notes on possible higher level morphological synapomorphies. Plant Systematics and Evolution, 229: 137–169. https://doi.org/10.1007/s006060170009
  20. Caputo P., Cozzolino S. 1994. A cladistic analysis of Dipsacaceae (Dipsacales). Plant Systematics and Evolution, 189: 41–61.
  21. Caputo P., Cozzolino S., Moretti A. 2004. Molecular phylogenetics of Dipsacaceae reveals parallel trends in seed dispersal syndromes. Plant Systematics and Evolution, 246: 163–175. https://doi.org/10.1007/s00606-004-0154-y
  22. Carlson S.E., Mayer V., Donoghue M.J. 2009. Phylogenetic relationships, taxonomy, and morphological evolution in Dipsacaceae (Dipsacales) inferred by DNA sequence data. Taxon, 58(4): 1075–1091. https://doi.org/10.1002/tax.584003
  23. Clarke G., Jones M.R. 1981. The Northwest European pollen flora. Dipsacaceae. Review of Palaeobotany and Palynology, 33: 1–25. [Reprinted in: The Northwest European pollen flora, vol. 3, parts 21–28. Eds W. Punt, G.C.S. Clarke. Amsterdam, etc.: Elsevier Sci. Publ. Company, pp. 21–25].
  24. Donoghue M.J., Olmstead R.G., Smith J., Palmer J.D. 1992. Phylogenetic relationships of Dipsacales based on rbcL sequences. Annals of the Missouri Botanical Garden, 79: 333–345. https://doi.org/10.2307/2399772
  25. Erdtman G. 1952. Pollen morphology and plant taxonomy. Angiosperms. Stockholm: Almqvist & Wiksell, 539 pp. https://doi.org/10.1080/11035895209453507
  26. Faegri K., Iversen J. 1964. Textbook of pollen analysis. Oxford: Blackwell, 237 pp.
  27. Gerasimenko N.P. 1997. Arkheologicheskiy almanakh. (Donetsk), 6: 3–64.
  28. Gerasimenko N.P., Korzun Yu.L., Ridush B.T. 2014. Fizychna heohrafiya ta heomorfolohiya, 2(74): 68–74.
  29. Grichuk V.P. 1972. Osnovnye etapy istorii rastitelnosti yugo-zapada Russkoy ravniny v pozdnem pleystotsene. In: Palinologiya pleystotsena. Moscow: Nauka, pp. 9–53.
  30. Grichuk V.P. 1989. Istoriya flory i rastitelnosti Russkoy ravniny v pleystotsene. Moscow: Nauka, 183 pp.
  31. Gubonina Z.P. 1975. Palinologicheskie issledovaniya osnovnykh gorizontov lessov i iskopaemykh pochv yuzhnoy chasti Russkoy ravniny. In: Problemy regionalnoy i obshchey paleogeografii lessovykh i periglyatsialnykh oblastey. Moscow, pp. 43–59.
  32. Halbritter H. 2016. Dipsacus fullonum. In: PalDat – a palynological database. Available at: https://www.paldat.org/pub/Dipsacus_fullonum/301814 (Accessed 17 July 2018).
  33. Halbritter H., Svojtka M. 2016. Dipsacus laciniatus. In: Pal-Dat – a palynological database. Available at: https://www.paldat.org/pub/Dipsacus_laciniatus/301809 (Accessed 17 July 2018).
  34. Kotov M.I. 1961. Dipsacaceae. In: Flora URSR, vol. 10. Ed. M.I. Kotov. Kyiv: Vyd-vo AN URSR, pp. 339–379.
  35. Kremenetskiy K.V. 1991. Paleoekologiya drevneyshikh zemledeltsev i skotovodov Russkoy ravniny. Moscow: Nauka, 193 pp.
  36. Kupriyanova L.A., Aleshina L.A. 1972. Pyltsa i spory rasteniy flory evropeyskoy chasti SSSR, vol. 1. Leningrad: Nauka, 170 pp.
  37. Mabberley D.J. 1997. The plant-book: a portable dictionary of the vascular plants. 2nd ed. Cambridge: Cambridge Univ. Press, 858 pp.
  38. Moore P.D., Webb J.A. 1983. An illustrated guide to pollen analysis. London, etc.: Hodder and Stoughton, 133 pp.
  39. Mostafa E.-N., Sedigheh N.-S., Rosa E. 2017. Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran. Bangladesh Journal of Plant Taxonomy, 24(2): 129–136. https://doi.org/10.3329/bjpt.v24i2.35108
  40. Mosyakin S.L., Fedoronchuk M.M. 1999. Vascular plants of Ukraine. A nomenclatural checklist. Kiev, xxiii + 345 pp.
  41. Pashkevich G.A. 1977. Palinologicheskoe issledovanie razreza stoyanki Korman IV. In: Mnogosloynaya paleoliticheskaya stoyanka Korman IV. Moscow: Nauka, pp. 105–111.
  42. Pashkevich G.A. 1987. Palinologicheskaya kharakteristika otlozheniy mnogosloynoy stoyanki Molodova V. In: Mnogosloynaya paleoliticheskaya stoyanka Molodova V. Lyudi kamennogo veka i okruzhayushchaya sreda. Moscow: Nauka, pp. 141–151.
  43. Perveen A., Qaiser M. 2011. Pollen flora of Pakistan. Dipsacaceae. Pakistan Journal of Botany, 43(6): 2825–2827.
  44. Punt W., Hoen P.P., Blackmore S., Nilsson S., Le Thomas A. 2007. Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology, 143: 1–81. http://doi.org/10.1016/j.revpalbo.2006.06.008
  45. Reveal J.L. 2012. An outline of a classification scheme for extant flowering plants. Phytoneuron, 2012-37: 1–221. Available at: https://www.phytoneuron.net/PhytoNMagnoliidae.pdf
  46. Simakova A.G., Puzachenko A.Yu. 2008. Rastitelnost v maksimalnoe pokholodanie poslednego oledeneniya (LGM) (‹=24.0–›=17.0 kyr BP). In: Evolyutsiya ekosistem Evropy pri perekhode ot pleistotsena k golotsenu (24–8 tys. l. n.). Eds A.K. Markova, T. Van Kolfekhoten. Moscow: Tov. nauch. izd. KMK, pp. 315–341.
  47. Sirenko E.A. 2017. Palinostratigrafiya kontinentalnikh verkhnepliotsenovykh–nizhneneopleystotsenovykh otlozheniy yuzhnoy chasti Vostochno-Evropeyskoy platformy. Ed. P.F. Gozhik. Kiev: Naukova Dumka, 166 pp.
  48. Stuchlik L., Kvavadze E.V. 1995. On the problem of actuopalynology in the Carpathians and Caucasus. Acta Palaeobotanica, 35: 73–83.
  49. Takhtajan A.L. 1987. Sistema magnoliofitov. Leningrad: Nauka, 439 pp.
  50. Takhtajan A.L. 1997. Diversity and classification of flowering plants. New York: Columbia Univ. Press, 663 pp.
  51. Takhtajan A. 2009. Flowering Plants. Dordrecht: Springer, xlv + 871 pp. https://doi.org/10.1007/978-1-4020-9609-9
  52. Thiers B. 2008–onward (continuously updated). Index Herbariorum: A global directory of public herbaia and associated staff. New York Botanical Garden's Virtual Herbaium. Available at: ttp://sweetgum.nybg.org/science/ih/(Accessed 03 January 2019).
  53. Tokarev P.I. 2002. Morfologiya i ultrastruktura pyltsevykh zeren. Moscow: KMK Scientific Press, 51 pp.
  54. Tsymbalyuk Z.M., Bezusko L.H. 2017a. Ukrainian Botanical Journal, 74(2): 122–130. http://doi.org/10.15407/ukrbotj74.02.122
  55. Tsymbalyuk Z.M., Bezusko L.H. 2017b. Ukrainian Botanical Journal, 74(3): 224–232. https://doi.org/10.15407/ukrbotj74.03.203
  56. Tsymbalyuk Z.M., Bezusko L.H. 2017c. Ukrainian Botanical Journal, 74(6): 539–547. https://doi.org/10.15407/ukrbotj74.06.539
  57. Tsymbalyuk Z.M., Bezusko L.H., Nitsenko L.M. 2018. Ukrainian Botanical Journal, 75(3): 248–259. https://doi.org/10.15407/ukrbotj75.03.248
  58. Tsymbalyuk Z.M., Mosyakin S.L. 2013. Atlas of pollen grains of representatives of Plantaginaceae and Scrophulariaceae. Kyiv: Nash Format, 276 pp. https://doi.org/10.13140/RG.2.2.16968.11527
  59. Turland N.J., Wiersema J.H., Barrie F.R., Greuter W., Hawksworth D.L., Herendeen P.S., Knapp S., Kusber W.-H., Li D.-Z., Marhold K., May T.W., McNeill J., Monro A.M., Prado J., Price M.J., Smith G.F. (eds). 2018. International Code of Nomenclature for Algae, Fungi, and Plants (Shenzhen Code) adopted by the Nineteenth International Botanical Congress. Shenzhen, China, July 2017. Regnum Vegetabile, 159: i–xxxviii + 1–254. https://doi.org/10.12705/Code.2018
  60. Vinokurova L.V. 1959. Palinologicheskie dannye k sistematike semeystv Dipsacaceae i Morinaceae. In: Problemy botaniki, vol. 4. Moscow; Leningrad: Izdvo AN SSSR, pp. 51–67.
  61. Zhang W.-H., Chen Z.-D., Li J.-H., Chen H.-B., Tang Y.-C. 2003. Phylogeny of the Dipsacales s. l. based on chloroplast trnL-F and ndhF sequences. Molecular Phylogenetics and Evolution, 26: 176–189. https://doi.org/10.1016/S1055-7903(02)00303-2
  62. Zubets R.Ya. 1971. Ukrainian Botanical Journal, 28(2): 192–198.