Ukr. Bot. J. 2014, 71(1): 78–82 https://doi.org/10.15407/ukrbotj71.01.078Plant Physiology, Biochemistry, Cell and Molecular Biology
Rhizogenesis of Arabidopsis thaliana wild type and scr mutant in vitro
Bulavin I.V.- M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv
Abstract
Two models of rhizogenesis in vitro, in the callus culture and leaf explants of Arabidopsis thaliana (L.) wild type (cv. Columbia) and scr mutant, were investigated. It was shown that roots formed de novo had a cap and all growth zones, similarly to those in embryonal roots. The appearance of adnated roots in the callus culture was noted. Based on the obtained data, the model of rhizogenesis from leaf explants is recommended for using in the experimental research in the field of gravitational and space biology.
Keywords: Arabidopsis thaliana (Col–0), scr mutant, callus, leaf explant, rhizogenesis
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