Prospects of cultivation of Anacanptis morio (L.) R.M. Bateman, Pridgeon et M.W. Chase (Orchidaceae Juss.) in Ukraine are considered. Methods of aseptic breeding of A. morio were developed; conditions for the plant sterilization and cultivation in vitro as well as nutrient media were selected and vital plant material was produced. Parameters for growing A. morio under primary culture conditions were determined. Cultivation of A. morio is proposed for the ex situ conservation of the species.

Keywords: orchids, Anacamptis morio, sterilization, aseptic propagation, cultivation, conservation

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