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Ukr. Bot. J. 2020, 77(1): 56–63
Biotechnology, Physiology and Biochemistry

Cultural and morphological characteristics of the species of Pholiota (Strophariaceae, Basidiomycota) on agar nutrient media

Regeda L.V., Bisko N.A.

The article provides cultural and morphological characteristics of vegetative mycelium on agar nutrient media of eight species (18 strains) of the genus Pholiota from the IBK Mushroom Culture Collection of the M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine. The strains were cultivated on two different media, malt extract agar (MEA) and glucose-peptone-yeast agar (GPYA), at pH 6.0 and at the temperature 26±1 ºC. It has been shown that cultural characteristics of the colony morphology and the rate of radial growth depend on nutrient media composition and biological properties of the strains. Most strains formed white colonies, later becoming light yellow or from orange to dark brown. Some of the species were able to form strands and allocate exudates. A slow rate of radial growth (up 0.52±0.08 to 2.24±0.18 mm/day) for all strains of the studied Pholiota species was noticed. The highest rate of radial growth for most of the studied strains was observed on glucose-peptone-yeast agar.

Keywords: growth rate, morphology, nutrient agar media, vegetative mycelium, Pholiota

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  1. Badalyan S.M., Barkhudaryan A., Rapior S. 2019. Medicinal Mushrooms Recent Progress in Research and Development. Singapore: Springer Nature Pte Ltd., 419 pp.
  2. Badalyan S.M., Gharibyan N.G. 2017. Characteristics of mycelial structures of different fungal collections. Yerevan: YSU Press, 176 pp.
  3. Belova N.V. 2004. Mycology and phytopathology, 38(2): 1–7.
  4. Bisko N.A., Babitskaya V.G., Buchalo A.S., Krupoderova T.A., Lomberg M.L., Mykhaylova O.B., Puchkova T.A., Solomko E.F., Shcherba V.V. 2012. Biological features of medicinal macromycetes in culture, vol. 2. Ed. S.P. Wasser. Sumy: Liaposchenko O.V., 459 pp.
  5. Bisko N.A., Lomberg M.L., Mykchaylova O.B., Mytropolska N.Yu. 2018. Conservation of biotechnological important species diversity and genetic resource of rare and endangered fungi of Ukraine. Plant & Fungal Research, 1(1): 18–27.
  6. Bisko N.A., Lomberg M.L., Mytropolska N.Yu., Mychaylova O.B. 2016. The IBK Mushroom Culture Collection (Kyiv, M.G. Kholodny Institute of Botany, National Academy of Science of the Ukraine). Kyiv: Alterpress, 120 pp.
  7. Buchalo A.S. 1988. Higher edible Basidiomycetes in pure culture. Kiev: Naukova Dumka, 144 pp.
  8. Buchalo A.S., Mykchaylova O.B., Lomberg M.L., Wasser S.P. 2009. Microstructures of vegetative mycelia of macromycetes in pure cultures. Eds P.A. Volz, E. Nevo, Kyiv: Alterpress, 224 pp.
  9. Cho Y.-H., Kong W.-S., Kim G.-H., Jhune C.-S., You C.-H., Yoo Y.-B., Kim K.-H. 2003. Analysis of cultural characteristics and phylogenic relationship of collected strains of Pholiota species. Mycrobiology, 31(4): 200–204.
  10. Deng P., Zhang G., Zhou B., Lin R., Jia L., Fan K., Liu X., Wang G., Wang L., Zhang J. 2011. Extraction and in vitro antioxidant activity of intracellular polysaccharide by Pholiota adiposa SX-02. Journal of Bioscience and Bioengineering, 111(1): 50–54.
  11. Dulger B. 2004. Antimicrobial activity of the macrofungus Pholiota adiposa. Fitoterapia, 75: 395–397.
  12. Dyakov M.Yu., Kamzolkina O.V., Shtaer O.V., Bisko N.A., Poedinok N.L., Michailova O.B., Tikhonova O.V., Tolstikhina T.E., Vasil'eva B.F., Efremenkova O.V. 2011. Morphological characteristics of natural strains of certain species of Basidiomycetes and biological analysis of antimicrobial activity under submerged cultural conditions. Microbiology, 80(2): 274–285.
  13. Gizaw B. 2015. Cultivation and yield performance of Pholiota nameko on different agro industrial wastes. Academia Journal of Food Research, 3(3): 32–42.
  14. Lomberg M.L. 2005. Medicinal macromycetes in surface and deep culture: Cand. Sci. Diss. Abstract. Kyiv, M.G. Kholodny Institute of Botany NAS of Ukraine, 7 pp.
  15. Mykchaylova O.B., Lomberg M.L., Bisko N.A. 2019. Verification and screening of biotecnologically valuable macromycetes species in vitro. In: Development of Modern Science: the Experience of European Countries and Prospects for Ukraine. Riga: Baltija Publishing, pp. 354–375.
  16. Regeda L.V., Bisko N.A. 2019. Micromorphological characteristics of the species of Pholiota (Strophariaceae, Basidiomycota) in pure culture. Ukrainian Botanical Journal, 76(2): 114–120.
  17. Stalpers J.A. 1978. Identification of wood-inhabiting Aphyllophorales in pure culture. Studies in Mycology, 16: 1–248.
  18. Stamets P. 2000. Growing Gourmet and Medicinal Mushrooms. 3rd revised ed. Washington: Ten Speed Press, 614 pp.
  19. Wang C.R., Qiao W.T., Zhang Y.N., Liu F. 2013. Effects of adenosine extract from Pholiota adiposa (Fr.) Quel on mRNA expressions of superoxide dismutase and immunomodulatory cytokines. Molecules, 18: 1775–1782.
  20. Wasser S.P. 2010. Medicinal mushrooms Science: History, Current status, Future Trends and Unsolved Problems. International Journal of Medicinal Mushrooms, 12(1): 1–16.
  21. Zhang G.Q., Sun J., Wang H.X., Ng T.B. 2009. A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa. Acta Biochimica Polonica, 56(3): 415–421.