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Ukr. Bot. J. 2018, 75(2): 191–196
https://doi.org/10.15407/ukrbotj75.02.191
Plant Physiology, Biochemistry, Cell and Molecular Biology

The state of the population of Schizophyllum commune (Agaricales, Basidiomycota) in Feofaniya forest parcel

Boiko S.M.
Abstract

Population research allows to establish biological diversity and provides an opportunity to determine the status of genetic resources of the fungi and distribution of the gene pool across the species range. The analysis of biochemical markers (proteins) allows to establish the loci (allelic) variability within the species at the population level, as well as to define population boundaries and to provide screening of biotechnologically valuable properties. Many factors affect the structure of fungal populations, such as propagation, mutation, genetic drift, population size, system of vegetative and sexual compatibility. Enzyme systems of basidiomycetes at the population level in Ukraine are poorly studied. The aim of the research was to investigate the population status of the fungus Schizophyllum commune within Feofania forest parcel using polymorphic intracellular enzyme systems. The allelic variability of Schizophyllum commune in the territory of Feofaniya forest parcel were established. Eleven allelic variants for Cat, Got, Amy2 and Eg loci, two for endo -1,3(4)-β-glucanase (Eg) and glutamic oxaloacetic transaminase (Got) enzymes, three for α-amylase (Amy2) and four for catalase (Cat) were identified. The deviation from the Hardy-Weinberg equation for the Eg and Cat loci is likely to be due to the limited population size and a high percentage of inbreeding within it. A deficiency of heterozygotes of endo-1,3(4)-β-glucanase and catalase enzymes and a balance of homo- and heterozygotes for Amy2 and Got loci were observed. The data on the genetic status of the population of S. commune in Feofaniya indicate population decline which is an evidence of its certain isolation.

Keywords: Schizophyllum commune, population, Feofaniya, loci, allele, spectrum of endoisozymes

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References
  1. Bilay V.I. Metody eksperimentalnoy mikologii. Kiev: Naukova Dumka, 1982, 550 pp.
  2. Boiko S.M. Ukr. Bot. J., 2011, 68(4): 598–603.
  3. Boiko S.M. Ukr. Bot. J., 2015, 72(3): 252–256.
  4. Boiko S.M. Population structure of the wood-decay fungus Trichaptum abietinum (J. Dicks.) Ryvarden in the Carpathian National Nature Park (Ukraine). Biodiv. Res. Conserv., 2016, 43: 1–6. https://doi.org/10.1515/biorc-2016-0017
  5. Boiko S.M. Pool of endoglucanase Schizophyllum commune Fr.: Fr. (Basidiomycetes) on the territory of Ukraine. Acta Biologica Szegediensis, 2018, 62(1), in press.
  6. Cooke W. B. The genus Schizophyllum. Mycologia, 1961, 53: 575–599. https://doi.org/10.2307/3756459
  7. Garre V., Tenberge K.B., Eising R. Secretion of a fungal extracellular catalase by Claviceps purpurea during infection of rye: putative role in pathogenicity and suppression of host defense. Phytopathology, 1998, 88: 744–753. https://doi.org/10.1094/PHYTO.1998.88.8.744 https://www.ncbi.nlm.nih.gov/pubmed/18944879
  8. James T.Y., Porter D., Hamrick J.L., Vilgalys R. Evidence for limited intercontinental gene flow in the cosmopolitan mushroom Schizophyllum commune. Evolution, 1999, 53: 1665–1677. https://doi.org/10.1111/j.1558-5646.1999.tb04552.x https://www.ncbi.nlm.nih.gov/pubmed/28565469
  9. Lamb C., Dixon R.A. The oxidative burst in plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997, 48: 251–275. https://doi.org/10.1146/annurev.arplant.48.1.251 https://www.ncbi.nlm.nih.gov/pubmed/15012264
  10. Layne E. Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzymology, 1957, 3: 447–455. https://doi.org/10.1016/S0076-6879(57)03413-8
  11. Leung H., Nelson R. J., Leach J. E. Population structure of plant pathogenic fungi and bacteria. Adv. Plant Pathol., 1993, 10: 157–205.
  12. Manchenko G.P. Handbook of detection of enzymes on electrophoretic gels. CRC Press, 2003, 553 p.
  13. Maurice S., Skrede I., LeFloch G., Barbier G., Kauserud H. Population structure of Serpula lacrymans in Europe with an outlook to the French population. Mycologia, 2014, 106(5): 889–895. https://doi.org/10.3852/12-344 https://www.ncbi.nlm.nih.gov/pubmed/25239607
  14. Mezhzherin S.V., Mezhzherina Ya.A. Dopovidi NAN Ukrainy, 2000, 4: 192–196.
  15. Mezhzherin S.V., Mezhzherina D.S., Mezhzherin I.S. Naukoviy visnyk Uzhgorodskogo univ., 2013, 34: 92–95.
  16. Micales J.A., Bonde M.R. Isozymes: methods and applications. In: Molecular methods in plant pathology. CRC Press, Boca Raton, 1995, pp. 115–130.
  17. Milgroom M.G. Analysis of population structure in fungal plant pathogens. In: Disease Analysis Through Genetics and Biotechnology: Interdisciplinary Bridges to Improved Sorghum and Millet Crops. Eds J.F. Leslie, R.A. Frederiksen. Iowa State University Press, Ames., 1995, pp. 213–230.
  18. Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 1978, 89: 583–590.
  19. Ohm R.A., de Jong J.F., Lugones L.G., Aerts A., Kothe E., Stajich J.E., de Vries R.P., Record E., Levasseur A., Baker S.E., Bartholomew K.A., Coutinho P.M., Erdmann S., Fowler T.J., Gathman A.C., Lombard V., Henrissat B., Knabe N., Kües U., Lilly W.W., Lindquist E., Lucas S., Magnuson J.K., Piumi F., Raudaskoski M., Salamov A., Schmutz J., Schwarze F.W., van Kuyk P.A., Horton J.S., Grigoriev I.V., Wösten H.A. Genome sequence of the model mushroom Schizophyllum commune. Nature Biotechnology, 2010, 28(9): 957–963. https://doi.org/10.1038/nbt.1643 https://www.ncbi.nlm.nih.gov/pubmed/20622885
  20. Raper C.A. Schizophyllum commune, a model for genetic studies of the Basidiomycotina. In: G.S. Sidhu. Genetics of Plant Pathogenic Fungi. London: Academic Press, 1988, pp. 511–522. https://doi.org/10.1016/B978-0-12-033706-4.50038-4
  21. Takemoto S., Nakamura H., Imamura Y., Shimane T. Schizophyllum commune as a ubiquitous plant parasite. Japan Agricultural Research Quarterly, 2010, 44: 357–364. https://doi.org/10.6090/jarq.44.357
  22. Wojtaszek P. Oxidative burst: an early plant response to pathogen infection. Biochem. J., 1997, 322(3): 681–692. https://doi.org/10.1042/bj3220681 https://www.ncbi.nlm.nih.gov/pubmed/9148737 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1218243
  23. Yeh F.C., Yang R., Boyle T. POPGENE Version 1.32. Microsoft window-based freeware for population genetic analysis. Univ. Alberta. Center Intern. Forestry Res., 1999.