The comparative cytochemical analysis of the localization of monolignins (syringyl and guaiacyl) in epidermis, photosynthesizing parenchyma and wood vessels cell walls of submerged leaves of Myriophyllum spicatum L., Potamogeton pectinatus L. and P. perfoliatus L. was carried using laser scanning (confocal) microscopy. The images of quantitative distribution of syringyl and guaiacyl in the cell walls were obtained depending on the type of leaf tissue and plant species. Using PASCAL software, the increased content of monolignols was established in cell walls of xylem vessels and in interspaces between epidermal and parenchyma photosynthesizing cells. It was shown that certain polarity of S/G ratio in cells is characteristic for every species. The role of syringyl and guaiacyl in the cellular mechanisms of adaptation to natural water environment is discussed.

Keywords: hydrophytes, monolignols, laser-confocal microscopy, Myriophyllum spicatum, Potamogeton pectinatus, Potamogeton perfoliatus

Full text: PDF (Ukr) 1.89M

1. Adler E. Wood Science and Technology, 1977, 11, pp. 169–218. http://dx.doi.org/10.1007/BF00365615
2. Baucher M., Monties B., Van Montagu M., Boerjan W. Crit. Rev. Plant Sci., 1998, 17, pp. 125–197. http://dx.doi.org/10.1016/S0735-2689(98)00360-8
3. Boerjan W., Ralph J., Baucher M. Annu. Rev. Plant Biol., 2003, 54, pp. 519–546. http://dx.doi.org/10.1146/annurev.arplant.54.031902.134938
4. Christiernin M. Composition of Lignin in Outer Cell-Wall Layers: Thesis of Diss., Royal Institute of Technology, Division of Wood Chemistry and Pulp Technology, Stockholm, 2006, pp. 1–53.
5. Fengelb D., Wegener G. Wood chemistry, ultrastructure, reaction, Berlin; NewYork: Walter de Gruyter, 1984, 611 p.
6. Grisebach H. Naturwissenschaften, 1977, 64, pp. 619–625. http://dx.doi.org/10.1007/BF00440094
7. Halchar F.Z., Marol Ch., Berge O., Bangel Castro I., Prosser J., Balesdent J., Heuulin Th., Achouak W. JSME, 2008, 2, pp. 1221–1230.
8. Jackson M.B., Colmer T.D. Ann. Bot., 2005, 96, pp. 501–505. http://dx.doi.org/10.1093/aob/mci205
9. Leisola M., Pastinen O., Axe D.D. Lignin–Designed. In: BIO-Complexity, 2012, issue 3, pp. 1–11, available at: http://bio-complexity.org/ojs/index.php/main/article/view/BIO-C.2012.3/ BIO-C.2012.3.
10. Little S.T. Adaptation and acclimaton of populatons of Ludwigia repens to growth in high- and lower-CO₂ springs: A Diss. pres. to the graduate school of the Univ. of Florida for the degree of PhD, USA: Univ. Florida, 2003, pp. 1–157.
11. Menden B., Kohlhoff M., Moerschbacher B.M. Phytochemistry, 2007, 68, pp. 513–529. http://dx.doi.org/10.1016/j.phytochem.2006.11.011
12. Monties B. Polymer Degrad. Stabil., 1998, 59, pp. 53–64. http://dx.doi.org/10.1016/S0141-3910(97)00166-3
13. Nedukha O., Kordyum E. The participation of cell wall polysaccharides in cellular mechanisms of leaf tolerance to nature flooding of plant. In: Plant Functioning under environmental stress: IX Inter. Conf., Kraków, 2013, pp. 137–152.
14. Nedukha O.M. Heterophylly in Plants, Kyiv: Alterpress, 2011, 198 p.
15. Vartapetian B, Andreeva I., Maslova I., Davtian N. Agrochimica, 1970, 15, pp. 1–19.
16. Vartapetian B., Andreeva I., Generozova I. Ann. Bot., 2003, 91, pp. 155–172. http://dx.doi.org/10.1093/aob/mcf244
17. Weng J.-K., Akiyama T., Bonawitz N.D., Li X., Ralph J., Chapple C. Plant Cell, 2010, 22(4): 1033–1045. http://dx.doi.org/10.1105/tpc.109.073528
18. Wuyts N., Lognay G., Swennen R., De Waele D. Secondary metabolites in roots and implications for nematode résistance in banana (Musca sp.). In: Proceed. of Inter. Symp. ‘Banana Root System: towards a better understanding for its productive management’ (San José, Costa Rica), San José: IPGRI, 2003, pp. 238–246.