Botrychium multifidum (S.G. Gmel.) Rupr. is a rare species included in the Red Data Book of Ukraine (2009). Due to analysis of the literature data, critical revision of the herbarium specimens and field studies of the authors, this species has been registered in Ukraine over 90 times. Botrychium multifidum is found to occur in 16 regions of Ukraine, e.g. Kyiv (15 localities), Lviv (14), Kharkiv (11), Zhytomyr and Chernihiv (9 localities in each), Ivano-Frankivsk and Sumy (7 localities in each), Volyn, Transcarpathian, Rivne and Chernivtsi (4 localities in each), Ternopil (2), Vinnitsa, Poltava, Kherson and Cherkasy (only one locality in each). The new localities of the species in Transcarpathian, Lviv, Kharkiv and Chernihiv Regions from herbarium materials (LWS, LE) as well as collections of the authors from Volyn Region are reported.
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